MCQ Bank
Which staining technique involves controlled thermal denaturation for highlighting telomeric regions?
- A) C-banding
- B) Q-banding
- C) T-banding
- D) R-banding
The primary staining agent used in T-banding to highlight telomeric regions?
- A) Giemsa stain
- B) Quinacrine mustard
- C) Acridine orange
- D) Hoechst 33258
How are chromosomes arranged in a karyotype?
- A) Based on size and shape
- B) Randomly
- C) Based on DNA content
- D) Alphabetically
Why is it important to avoid self-complementarity in PCR primers?
- A) It promotes primer specificity
- B) It has no impact on PCR
- C) It enhances PCR yield
- D) It reduces primer efficiency
Which of the following describes the purpose of cDNA synthesis?
- A) Editing genomic DNA
- B) Amplifying DNA sequences
- C) Generating a complementary DNA copy from RNA
- D) Replicating RNA molecules
Which blotting technique is commonly used to transfer proteins from a gel to a solid support membrane?
- A) Southern blotting
- B) Northern blotting
- C) Eastern blotting
- D) Western blotting
Which of the following is a crucial factor in primer design for PCR?
- A) Specificity for the target sequence
- B) Short length
- C) Low melting temperature
- D) High GC content
What is the purpose of a transfer buffer in blotting techniques?
- A) To dilute samples
- B) To denature proteins
- C) To facilitate the transfer of molecules from the gel to the membrane
- D) To amplify target molecules
cDNA is produced from
- A) Protein
- B) DNA
- C) tRNA
- D) mRNA
The primary objectives of isolation workflows are to stabilize RNA molecules, suppress _______, and optimize yield through appropriate storage and extraction techniques.
- A) Proteinase
- B) Transcriptase
- C) DNAses
- D) RNAses
What is the primary function of reverse transcriptase during cDNA synthesis?
- A) Proofreading DNA sequences
- B) Ligating DNA fragments
- C) Synthesizing DNA from RNA
- D) Synthesizing RNA from DNA