Which of the following is not an application of siRNA

• A) Cancer prevention, infections and developmental defects
• B) Easier than preparing knockout Used for partial knockdowns
• C) Determining peptide function
• D) Determining tRNA level

________ with RNase motif, is an enzyme of RNase III family
In humans, it is encoded by the DICER1 gene.

• A) polymerase
• B) DNase
• C) helicase
• D) dicerase

The population of mRNAs expressed by a genome at any given time is called

• A) Proteome
• B) Metablome
• C) Genome
• D) Transcriptome

RISC is a large (~500-kDa) RNA multi-protein complex, which triggers ____ degradation in response to ____

• A) DNA-histone
• B) mRNA-siRNA
• C) DNA-RNA
• D) protein-aminoacid

Small interfering RNA interferes with

• A) tRNA binding
• B) gene expression
• C)
• D)

The study of characteristics and regulation of the functional RNA transcript population of a cell/s or organism at a specific time is:

• A) Genomics
• B) Proteomics
• C) Lipidomics
• D) Transcriptomics

The population of _______ expressed by a genome at any given time is transcriptomics

• A) tRNA
• B) mRNA
• C) all of these
• D) rRNA

Dicer cleaves double-stranded RNA (dsRNA) and pre-microRNA into short double-stranded RNA fragments called

• A) • siRNA
• B) • dsRNA
• C) • snRNA
• D) • ssRNA

siRNA’s better target for efficient work is

• A) 3’ UTR
• B) 3’ AGT
• C) 5’ AGT
• D) 5’ UTR

Unwinding of double-stranded siRNA occurs by

• A) ATP dependent polymerase
• B) ATP dependent helicase
• C) ATP independent polymerase
• D) ATP independent helicase

30% of mammalian mRNAs are regulated by ______.

• A) Ribosomal RNA
• B) Nuclear RNA
• C) miRNAs
• D) Transfer RNA

RISC is a _______ kDa, RNA-multiprotein complex

• A) 500
• B) 50
• C) 5 x 10
• D) 5

Helicos
Pacific Biosciences
Oxford nanopore
The are related with what?

• A) 3GS
• B) PCR
• C) Gene cloning
• D) 2d PAGE

Virtual Terminator in Helicos prevents incorporation of multiple __________ per cycle

• A) histone
• B) nucleic acid
• C) peptides
• D) nucleotides

How many types of semi conductor chips:

• A) 7
• B) 5
• C) 3
• D) 10

Drosha and Dicer are ____ enzymes.

• A) RNase II
• B) DNase III
• C) DNase II
• D) RNase III

RISC mediates which cellular function:

• A) • Degrades mRNA
• B) • Starts Transcription
• C) • Degrades tRNA
• D) • Helps translation

Assigning identities and functions to sequences within the genome is called ________.

• A) Genome Sequencing
• B) Genomic evaluation
• C) cDNA formation
• D) Genome annotation

Transcription factors bind to specific __________.

• A) cDNA sequences
• B) RNA sequences
• C) Protein sequences
• D) DNA sequences

Mouse and human genomes each contains approximately ______ protein coding genes.

• A) 23000
• B) 10000
• C) 25000
• D) 5000