Which of the following practices should be utilized when working in a biological safety cabinet?

• A) Disinfect work surface before and after work
• B) Disinfect all items which go inside and out of BSC
• C) Do not store any item in BSC
• D) All of the above

First discovered, Type II restriction endonuclease was

• A) EcoR1
• B) EcoK
• C) Hind 1
• D) Hind11

Transport of infectious materials require ___________

• A) International regulations
• B) IATA shipping
• C) Packaging
• D) All given

Micro incinerators are heated by _____________ or electricity

• A) Fire
• B) All given
• C) Gas
• D) Heat

An individual can learn about the hazards of specific chemicals by?

• A) Reading the Material Safety Data Sheet
• B) Talking to friends about it
• C) Reading science magazines
• D) Asking a co-worker

Which of the following enzyme is used to cut DNA in rDNA technology?

• A) Phosphatase
• B) ribonuclease
• C) Ligase
• D) Restriction enzymes

Work with high concentrations of ___________ is the risk factor in high risk TB labs

• A) Cocci
• B) E.Coli
• C) Bacilli
• D) Spirilla

Bio safety cabinets are used not only to protect the worker but also

• A) protect the lab environment from the microorganisms
• B) enable eating and drinking in the lab
• C) absolve off the lab workers from wearing lab coats
• D) prevent the lab from overheating

Hazardous chemicals follow the ____________ route of exposure

• A) Needle sticks
• B) Ingestion
• C) Inhalation
• D) All given

Gene cloning refers to the

• A) Both A and B
• B) Production of large no. of copies of the gene being cloned
• C) Production of asexual progeny from single cell
• D) None of these

DNA extraction of TB should be performed in

• A) Low risk Tb labs
• B) High risk Tb labs
• C) Minimums risk Tb labs
• D) Moderate risk Tb labs

Which of the following statements about Personal Protective Equipment (PPE) are correct?

• A) PPE should be worn and stored only inside the laboratory
• B) Employees utilizing PPE should be properly trained
• C) PPE should be chosen based upon the work being completed
• D) All of the above

Pathogenic strains increase______________ of GMOs

• A) Resistance
• B) Half-life
• C) Production
• D) Virulence

Restriction enzymes

• A) All of the above
• B) Protect bacteria from viral infection
• C) cut DNA in a staggered fashion
• D) cut DNAs producing a blunt end

Emergency services include ______________

• A) Emergency equipment
• B) First aid
• C) All given
• D) Adress/Phone numbers

In order to insert a foreign gene into a plasmid, both must

• A) be cut by the same restriction enzyme
• B) have identical DNA sequence
• C) be of the same length
• D) originate from the same type of cell

In vacuum line protection_____________ prevent the passage of microbes

• A) Chemicals
• B) Autoclave
• C) Radiations
• D) Filters

Material Safety Data Sheets provide information about

• A) All of the above
• B) Emergency information
• C) Hazards about a specific chemical
• D) Information on chemical reactions

On the multi-colored chemical label, White represents

• A) Fire Hazard
• B) Reactivity Hazard
• C) Health Hazard
• D) Special Hazards

In vacuum line protection flask contain the _____________

• A) Chlorine
• B) Formaldehyde
• C) Ethanol
• D) Disinfectants