What key feature sets lipids apart from other compounds?

• A) Hydrophobic nature
• B) Solubility in water
• C) Insolubility in nonpolar solvents
• D) Presence of hydrocarbons

In what manner can the detection of lipids be achieved through the utilization of the Spot Test?

• A) Greasy spot on filter paper
• B) Formation of bubbles
• C) Change in pH
• D) A colored reaction

Different amino acids migrate at different rates on the paper because of difference in their _______.

• A) Alpha carbon
• B) Amino group
• C) R group
• D) Carboxylic group

In saponification, oil or fat is hydrolyzed with alkali that results in the formation of

• A) Glycerol and carboxylic acid
• B) Glycerol and salt of fatty acids
• C) Acid and ester
• D) Alcohol

To prepare 1% starch solution, _____ of starch dissolved in 100ml of distilled water.

• A) 1g
• B) 0.1g
• C) 10g
• D) 5g

Enzyme activity is a measure of

• A) Quantity of inactive enzymes present
• B) Quality of enzymes present
• C) Quantity of active enzymes present
• D) Quantity of enzymes present

Which conclusion can be drawn from a sample's solubility in chloroform and insolubility in water during the Solubility Test?

• A) The sample is likely a nucleic acid.
• B) The sample is likely a lipid
• C) The sample is likely a carbohydrate
• D) The sample is likely a protein

Chromatography is an analytical method used for separation of different biomolecule on the basis of their

• A) Physiochemical properties
• B) All of the above
• C) Chemical properties
• D) Physical properties

Rf is simply the distance that a biomolecule travelled through the filter paper divided by the distance that the ______ travelled through the paper.

• A) Solvent
• B) Sample
• C) Biomolecule
• D) Solute

The composition of the chromatography solvent used in the procedure?

• A) Butan-1-ol: Acetic acid: Water in 75:10:15 ratio.
• B) Butan-1-ol: Acetic acid: Water in 60:15:25 ratio.
• C) Butan-1-ol: Acetic acid: Water in 1:1:1 ratio.
• D) Butan-1-ol: Acetic acid: Water in 40:30:30 ratio.

The saponification number is the number of milligrams of potassium hydroxide required to neutralize the fatty acids resulting from the complete hydrolysis of _______.

• A) 1g of carbohydrate
• B) 1g of fat
• C) 1g of glucose
• D) 1g of protein

The role of drying the chromatography paper in a drying oven after spraying with Ninhydrin solution?

• A) To reduce the size of the amino acid spots.
• B) To develop the color reaction of amino acids.
• C) To accelerate the solvent migration
• D) To prevent the solvent from evaporating.

By what specific feature are lipids characterized, leading to their insolubility in water and solubility in nonpolar solvents

• A) High molecular weight
• B) Presence of hydrocarbons
• C) Formation of bubbles
• D) Acidic nature

In the Spot Test for lipids, what kind of spot formation indicates the presence of lipids?

• A) Formation of crystals
• B) Dark color
• C) Change in temperature
• D) Translucent and greasy spot

Which of the following options correctly characterizes a common structural feature of triglycerides?

• A) Unique branching pattern
• B) Shared hydrophobic tails
• C) Singular polar head
• D) Consistent ring structure

Saponification literally means

• A) Soap Formation
• B) Soap Production
• C) Soap Making
• D) Soap Extraction

Why is Ninhydrin spray used in amino acid paper chromatography?

• A) To neutralize the chromatography solvent.
• B) To detect the location of amino acids
• C) To dry the chromatography paper quickly
• D) To enhance the capillary action of the solvent.

Which of the following terms best describes the compound that serves as a precursor for the synthesis of steroid hormones?

• A) Catalyst for steroid production
• B) Building block for steroid hormones
• C) Inhibitor of steroid formation
• D) Initiator of steroid synthesis

During the Saponification test, what is formed as a result of the hydrolysis of oil or fat with alkali?

• A) Fatty acids
• B) Soap
• C) Bubbles
• D) Glycerol

The degree of movement of biomolecule up the paper is associated to its relative affinity for paper that is __________ in nature

• A) None of the above
• B) Hydrophillic
• C) Hydrophobic
• D) Both