The human genome consists of ~ ……… base pairs.

• A) 3.2 million
• B) 2.3 million
• C) 2.3 billion
• D) 3.2 billion

PDB allows to search following 3D structures …………..

• A) All of these
• B) Experimentally determined
• C) Computed Structure Models
• D) CSM

…………… compares protein sequence against protein databases. (Choose most relevant option)

• A) blastp
• B) blastx
• C) blastn
• D) tblastx

In an alignment all sequences have ............. length.

• A) None of these
• B) Different
• C) Equal
• D) Multiple of 2

What is the main function of UniProt Metagenomic and Environmental Sequences (UniMES)?

• A) To store sequences which are recovered directly from environmental samples
• B) To maintain a vast collection of bioinformatics databases and services
• C) To provide comprehensive protein sequence and functional information
• D) To provide a reference set of clusters for all UniProtKB sequences

Which tool/database/software of the following is used to visualize and analyze the protein 3D structure

• A) Chimera
• B) PSIPRED
• C) Uniprot
• D) Blast

What does the atomic composition describe in Protparam results?

• A) The number of hydrogen bonds in the protein
• B) The count of different types of atoms present in the protein sequence
• C) The arrangement of atoms in the protein structure
• D) The molecular weight of the protein

The residues that do not fall within the favored region of the Ramachandran plot are termed:

• A) Ramachandran outliers
• B) Ramachandran intermediates
• C) Ramachandran inliers
• D) Ramachandran conformers

The isoelectric point (pI) of a molecule is characterized by:

• A) The pH where the molecule is neutral, having a net charge of zero
• B) The pH where the molecule has the lowest net charge
• C) The pH where the molecule has the highest net positive charge
• D) The pH where the molecule loses its structure

In Uniprot, Sequence Archive is a:

• A) None
• B) Non redundant archive
• C) Redundant archive
• D) Semi-redundant archive

In vivo half-life means ---- of a protein in the cell.

• A) Strength
• B) Replacement
• C) Replacement or degradation
• D) Degradation

______ is a short sequence that contains one or more conserved residues, which allow the protein to bind to a ligand and carry out a catalytic activity. Hint: InterPro

• A) Active site
• B) Conserved site
• C) PTM site
• D) Binding site

Which of the following statements about the global alignment algorithm is incorrect? (Recall the concepts of Needleman-Wunsch Algorithm)

• A) Score can be negative
• B) First row and First column are set to Zero “0”
• C) In trace-back step, beginning is with the cell at the lower right of the matrix, and it ends at top left cell
• D) In initialization step, the first row and first column are subject to gap penalty

Which of the following statement is not true about BLAST program?

• A) The programs are organized based on the type of nucleotide sequences OR nucleotide sequence to be translated
• B) BLAST is not based on heuristic searching methods
• C) The programs are organized based on the type of query sequences
• D) The BLAST web server has been designed in such a way to simplify the task of program selection

Command line versions of the ClustalW programs are available for which platform?

• A) All of these
• B) UNIX
• C) Window base PCs
• D) Macintosh

In I-TASSER results, the lowest value of predicted solvent accessibility indicates:

• A) Hydrophobic residues
• B) Surface residues
• C) Buried residues
• D) Exposed residues

ClustalW is a recent version of Clustal with W standing for ...................

• A) Wiping
• B) Weighting
• C) Weakening
• D) Winding

What is the main function of UniProt Archive (UniParc)?

• A) To provide compressive protein sequence and functional information
• B) To capture all publicly available protein sequence data and contains all the protein sequences from the main publicly available protein sequence databases
• C) To maintain a vast collection of bioinformatics database and services
• D) To provide a reference set of clusters for all UniProtKB sequences

If the instability index is higher than 40, it indicates:

• A) The protein is stable
• B) The protein is unstable
• C) The protein is in a denatured state
• D) The protein is flexible

GenBank, the nucleic acid sequence database is maintained by

• A) European Molecular Biology Laboratory (EMBL)
• B) UniProt/ Swiss-Prot Database
• C) DNA Database of Japan (DDBJ)
• D) National Centre for Biotechnology Information (NCBI)