MCQ Bank
What is the composition of LB medium?
- A) 1 % NaCl, 1 % Tryptone, 0.5 %Yeast Extract
- B) 0.5 % NaCl, 1 % Tryptone, 1 %Yeast Extract
- C) 1 % NaCl, 0.5 % Tryptone, 0.5 %Yeast Extract
- D) 1 % NaCl, 1 % Peptone, 0.5 %Yeast Extract
How the microorganisms are killed by dry heat?
- A) Inactivation of RNA
- B) Oxidation effects
- C) Inactivation of DNA
- D) Coagulation of proteins
What is the wavelength range of ultraviolet radiations?
- A) 100-200 nm
- B) 100-400 nm
- C) 200-265 nm
- D) 400-700 nm
Which of the following is a limitation of autoclaving?
- A) It cannot be used to sterilize culture media
- B) It lacks the ability to inactivate viruses
- C) It lacks the ability to kill endospores
- D) It destroys some heat labile materials
Which of the following is the preferred method for the sterilization of glass petri plates?
- A) Moist heat sterilization
- B) Chemical method of sterilization
- C) Mechanical method of sterilization
- D) Dry heat sterilization
How the microorganisms are killed by using alcohols?
- A) Alkylation of DNA
- B) Addition of halogens
- C) Denaturation of proteins
- D) Oxidization of the cellular components
Which of the following can be sterilized using dry heat sterilization?
- A) Plastic petri plates
- B) Glass pipettes
- C) Culture media
- D) Rubber gloves
Which of the following is NOT an example of ionizing radiations?
- A) γ-rays
- B) UV rays
- C) High energy electron beam
- D) X-rays
Which of the following is used as carbon source in Malt Extract Agar?
- A) Lactose
- B) Dextrose
- C) Peptone
- D) Yeast extract
Which of the following is NOT true for moist heat sterilization?
- A) It is done using an autoclave
- B) It may release toxic fumes
- C) It kills both bacteria and viruses
- D) It may cause metal to rust
What are the conditions required for performing ultra-high temperature (UHT) pasteurization?
- A) 63°C for 30 minutes
- B) 135°C to 140°C for 2 to 4 seconds
- C) 72°C to 74°C for 15 to 20 seconds
- D) 57°C - 68°C for 15 minutes
Which one of the following is an inorganic nitrogen source used in fermentation medium?
- A) Yeast Extract
- B) Ammonia
- C) Urea
- D) Amino acid
Baffles are metal strips about ________of the vessel diameter.
- A) 1/25th
- B) 1/10th
- C) 1/20th
- D) 1/5th
Which of the following method is frequently used in hospitals and clinical laboratories for the sterilization of antibiotics?
- A) Filtration
- B) Physical method using radiations
- C) Dry heat sterilization
- D) Moist heat sterilization
Which of the following is NOT an advantage of submerged fermentation?
- A) Cheap process
- B) Downstream processing is easy
- C) Process parameters can be automated
- D) Uniform distribution of nutrient and waste products
Which one of the following is an organic nitrogen source used in fermentation medium?
- A) Urea
- B) Ammonia
- C) Ammonium sulfate
- D) Ammonium chloride
Which of the following is a basal medium?
- A) Mannitol salt agar
- B) Mannitol salt agar
- C) MacConkey agar
- D) Nutrient agar
The pore size of membrane filter is __________.
- A) 0.1-1 µm
- B) 0.1 -0.22 µm
- C) 0.45 -0.65 µm
- D) 0.22-0.45 µm
Which of the following represents generation time?
- A) the length of time it takes to reach the log phase
- B) the length of time of the exponential phase
- C) the time it takes to reach stationary phase
- D) the length of time it takes for a population of cells to double
Which of the following medium is used to determine the colony characteristics of bacteria?
- A) Nutrient agar medium
- B) YPD medium
- C) Potato dextrose agar medium
- D) LB broth medium