If DNA library is established using expression vectors, each individual clone can be expressed to yield a

• A) Dipeptide
• B) Monopeptide
• C) Nucleotide
• D) Polypeptide

ABC pathway for protein export and Type II secretion pathway are used for

• A) Protein trafficking
• B) DNA trafficking
• C) RNA trafficking
• D) Chromosome trafficking

____________________ depends upon transcription of appropriate gene, efficient translation of mRNA and in many cases, post-translational processing and compartmentalization of nascent polypeptide.

• A) Synthesis of genome
• B) Synthesis of functional protein
• C) Synthesis of functional RNA
• D) Synthesis of functional DNA

________ are simply plasmids designed for the cloning of very long segments

• A) Human artificial chromosomes
• B) Bacterial artificial chromosomes
• C) Viral artificial chromosomes
• D) Yeast artificial chromosomes

Vectors that can replicate and are maintained in two or more unrelated host organisms are called ____vectors

• A) expression
• B) shuttle
• C) host
• D) house keeping

Expression from a strong promoter can represent ________ of cloned gene product of total cell protein

• A) 20-50%
• B) 30-40%
• C) 20-40%
• D) 10-40%

YACs are the ______ used for large DNA fragments insertion

• A) Yeast artificial chromosomes
• B) Y-cloned artificial chromosomes
• C) Yok artificial chromosomes
• D) Y-linked autosomal clones

Foreign proteins in E. coli is

• A) submerged
• B) Stable
• C) Dissolved
• D) Instable

Vectors that can replicate and are stably maintained in two or more unrelated host organisms are called ____

• A) internal vectors
• B) shuttle vectors
• C) target vectors
• D) host specific vectors

A great advantage of hybridization for library screening is that it is

• A) Difficult
• B) Good
• C) Versatile
• D) Unique

It is much more convenient to use ________________, because these have a higher capacity and the efficiency of in vitro packaging

• A) bacteriophage simple insertion vectors
• B) Bacteriophage-ʎ insertion vectors
• C) Bacteriophage-ECol I insertion vectors
• D) Bacteriophage-ʎ deletion vectors

Gram-negative bacteria such as E. coli have a

• A) complex wall-membrane mitochondia
• B) Complex wall-membrane
• C) Difficult wall-membrane
• D) Simple wall-membrane

This is the process by which a particular DNA sequence compensates for a missing function in a mutant cell and thus restores the wild type phenotype.

• A) Functional complementation
• B) Functional genomics
• C) Non-Functional complementation
• D) Functional analysis

________ with specific primers could be used to isolate genes directly from genomic DNA

• A) SNPs
• B) PCR
• C) AFLP
• D) RFLP

Bacterial artificial chromosomes are used as_____

• A) plasmid recombinant vectors
• B) cloning vectors
• C) restriction enzymes
• D) protein docking molecules

_______ are the plasmids containing a fragment of ʎ DNA including ‘cos’ site

• A) phasmid
• B) M13
• C) none of these
• D) cosmid

In chromosome jumping, the DNA of interest is identified, cut into fragments with restriction enzymes and

• A) Breaks down
• B) Circularized
• C) Straighten
• D) Elongates

In Genomic DNA libraries, the appropriately sized random fragments can be produced by ______

• A) polymerases
• B) ligases
• C) lysozymes
• D) restriction endonucleases

In South-western and north-western blotting screening is carried out without using an antibody, by incubating the membranes with

• A) Radiolabelled single stranded DNA probe
• B) Unlabelled double stranded DNA probe
• C) Radiolabelled double stranded DNA probe
• D) Radiolabelled double stranded RNA probe

________ vectors are combination of plasmid and ʎ phage sequences

• A) M13
• B) Phasmid
• C) Cosmid
• D) Chloroplast