In Genomic DNA libraries, the appropriately sized random fragments can be produced by _________

• A) Lysozymes
• B) Polymerases
• C) Ligases
• D) Restriction endonucleases

Major screening strategies involve _______

• A) All of these
• B) Genetic methods
• C) Sequence-dependent screenings
• D) Screening expression libraries

cDNA is sequence which lacks ________ sequences.

• A) introns
• B) untraslated exons
• C) none of these
• D) exons

Genomic DNA library is constructed using _______ cloning vectors.

• A) low capacity
• B) high capacity
• C) low copy number
• D) low copy and capacity

BamHI is an enzymes used in the creation of ______ by using the phage-ʎ vector EMBL3A.

• A) rRNA molecules
• B) Mutated DNA
• C) Protein molecules
• D) Genomic DNA library

The ________ with specific primers could be used to isolate genes directly from genomic DNA

• A) PCR
• B) SNPs
• C) AFLP
• D) RFLP

Synthesis of cDNA from RNA is done using _____

• A) inverse-PCR
• B) reverse-PCR
• C) qPCR
• D) RT-PCR

cDNA library is representative of the ___ population of the derived host

• A) Proteins
• B) DNA & RNA
• C) DNA
• D) RNA

For _____ libraries, the correct orientation can be achieved by self-priming method by adding linker molecule to double stranded cDNA

• A) genome expression
• B) cDNA expression
• C) protein expression
• D) mRNA expression

Researchers prefer to use cDNA rather than mRNA because__________

• A) All of the above
• B) RNAs are inherently less stable
• C) routine amplification is not present
• D) purifying RNA is difficult

It is much more convenient to use ________________, because these have a higher capacity and the efficiency of in vitro packaging.

• A) Bacteriophage-ECol I insertion vectors
• B) Bacteriophage-ʎ deletion vectors
• C) Bacteriophage-ʎ insertion vectors
• D) bacteriophage simple insertion vectors

If DNA library is established using expression vectors, each individual clone can be expressed to yield a _____________

• A) Dipeptide
• B) Nucleotide
• C) Polypeptide
• D) Monopeptide

A great advantage of hybridization for library screening is that it is ______________

• A) Good
• B) Unique
• C) Versatile
• D) Difficult

Functional complementation is also possible in __________________

• A) Transgenic animals and plants
• B) Artificial animals and plants
• C) Transgenic mitochondria
• D) Non-Transgenic animals and plants

Immunochemical screening involves the use of ________________

• A) Antibodies
• B) DNA
• C) PCR
• D) Antigens

To isolate specific clone, PCR is carried out with _____________

• A) universal primers
• B) simple primers
• C) gene-specific primers
• D) special primers

In chromosome jumping, the DNA of interest is identified, cut into fragments with restriction enzymes and ________________

• A) Circularized
• B) Straighten
• C) Breaks down
• D) Elongates

In South-western and north-western blotting screening is carried out without using an antibody, by incubating the membranes with ____________

• A) Radiolabelled single stranded DNA probe
• B) Radiolabelled double stranded RNA probe
• C) Radiolabelled double stranded DNA probe
• D) Unlabelled double stranded DNA probe

The _________________ is the process by which a particular DNA sequence compensates for a missing function in a mutant cell and thus restores the wild type phenotype.

• A) Functional complementation
• B) Non-Functional complementation
• C) Functional analysis
• D) Functional genomics

M13 is a filamentous _________ with single stranded DNA that replicates without killing its host.

• A) bacteriophage
• B) chloroplast
• C) mitochondrial
• D) plasmid