Trizole is an efficient method of RNA isolation, Trizole reagents promotes the:

• A) Degradation of RNases
• B) Precipitation of RNA
• C) Dissociation of Proteins and nucleic acids
• D) Deactivation of RNA

The distance of DNA migration in a gel during electrophoresis primarily depends upon?

• A) pH of the buffer
• B) DNA concentration
• C) Voltage
• D) Size of DNA

Which of the following is commonly involved in the protocol for isolating DNA using a commercial kit?

• A) Chromatography
• B) Electrophoresis
• C) Centrifugation
• D) PCR

The principle of Nanodrop spectrophotometer for nucleic acid quantification is based on?

• A) Light absorbance
• B) Resonance
• C) Electrophoresis
• D) Flourescence

Trizole in RNA extraction promotes

• A) Dissociation of Proteins and nucleic acids
• B) the degradation of RNases
• C) None of the options
• D) Precipitation of RNA

Which of the following step is performed to purify the RNA pellet during the RNA isolation by Trizol method?

• A) Chloform addition
• B) PCI precipitation
• C) Lysis
• D) Washing with ethanol

Which of the following is tracking dye in gel electrophoresis?

• A) Xylene blue
• B) Bromophenol Blue
• C) Crystal Violet
• D) Ethidium Bromide

What does a 260/280 ratio of 2.0 indicate in RNA quantification?

• A) Low RNA concentration
• B) Contaminated with DNA
• C) Contaminated with proteins
• D) Pure RNA sample

The kit method of DNA isolation can be used to extract DNA from?

• A) Cell cultures
• B) Blood sample
• C) All of the above
• D) Mamalian Tissue

In electrophoresis which DNA will move faster towards end

• A) 700bp
• B) 100bp
• C) 300bp
• D) 1Kb

What is the purpose of washing the DNA pellet with ethanol in the final steps?

• A) To increase DNA yield
• B) To remove remaining cell debris
• C) To isolate DNA from solution
• D) To improve DNA stability

Which dye is used in gel electrophoresis to visualize DNA bands under UV light?

• A) Ethidium bromide
• B) Bromophenol blue
• C) Methylene blue
• D) Crystal violet

Which of the following step is NOT involved in the DNA isolation using a commercial kit?

• A) DNA binding
• B) Cell lysis
• C) Phenol-chloroform separation
• D) DNA elution

DNA isolation from blood is done from which cells?

• A) Platelets
• B) Neutrophils
• C) WBCs
• D) RBCs

What is the charge on DNA that makes it move during gel electrophoresis?

• A) Both positive and negative charges
• B) DNA does not have nay charge
• C) Positive charge
• D) Negative charge

If we have DNA fragments of the following sizes, which fragment in gel electrophoresis will move faster?

• A) 100 bp
• B) 1 kb
• C) 500 bp
• D) 1000 bp

What determines the migration rate of DNA fragments in gel electrophoresis?

• A) Their function
• B) Their shape
• C) Their color
• D) Their size and charge

CTAB used in the DNA extraction from plant tissue, stands for?

• A) Cellular Tissue Absorption Buffer
• B) Cellulose Trisodium Absorption Buffer
• C) Cetyl-trimethyl-ammonium Bromide
• D) Chloroplast Treatment and Absorption Base

Why is RNase used in the CTAB-based DNA extraction from plant tissue?

• A) To increase DNA yield
• B) To prevent protein contamination
• C) To enhance RNA yield
• D) To prevent RNA contamination

Which of these dyes we could use to visualize DNA in agarose gel?

• A) Carmine
• B) Ethidium bromide
• C) Ethidium dichloride
• D) Crytal Voilet