Select a reason why is it necessary to ensure the proper pH of the liquid culture medium?

• A) To control the coloration of bacteria
• B) To optimize enzymatic activity
• C) To prevent bacterial growth
• D) To maintain the osmotic balance

Indicate which instrument that is used to separate particles suspended in liquid?

• A) Weighing balance
• B) Safety cabinet
• C) Centrifuge machine
• D) Autoclave

Select a type of bacteria that are unable to grow in artificial media?

• A) Anerobic bacteria
• B) Obligate parasites
• C) Aerobic bacteria
• D) Facultative parasite

Sterilization is also performed by holding materials in a bunsen burner flame until they become red hot. Which of the following material is sterilized by use of red heat?

• A) Petri plates
• B) Glass slides
• C) Inoculating loop
• D) Test Tubes

Among the following, select which one is an essential nutrient often required for bacterial growth in a liquid medium?

• A) Ultraviolet light
• B) Nitrogen
• C) Sound waves
• D) Electrical energy

Which of the following part of microscope is used to bring the object being viewed closer?

• A) Eyepiece
• B) Objective lens
• C) Stage controls
• D) Coarse Adjustment Knobs

Select the purpose of the UV germicidal lamp used in BSCs?

• A) To maintain a controlled temperature
• B) Provide additional lighting
• C) To sterilize the work surface and contents
• D) Sterilize the operator's hands

The purpose of sterilization is --------. Select the most suitable option.

• A) Recovery of products
• B) Purification of products
• C) Degradation of material
• D) Elimination of contamination

What is the magnification power of oil immersion lense?

• A) 100X
• B) 10X
• C) 40X
• D) 20X

An organization has developed the Biosafety Levels to handle hazardous biological materials in laboratories? Select which organization has developed biosafety levels?

• A) World Health Organization
• B) Clinical Laboratory Standard Institution
• C) Centers for Disease Control and Prevention
• D) Occupational Safety and Health Administration

Select among the following is considered as essential for visualizing microorganisms and their structures?(

• A) pH meter
• B) Incubator
• C) Microscope
• D) Spectrophotometer

Which of the following filters has pad like structure?

• A) Membrane filters
• B) MF-Millipore
• C) Glass filters
• D) Seitz filter

What was the pressure of the autoclave at which it was operated in your lab?

• A) 10psi
• B) 5psi
• C) 15psi
• D) 20psi

A device used for delivering minute amounts of solutions in microliters (file:///C:/Users/NAFEES~1.SAF/AppData/Local/Temp/msohtmlclip1/01/clip_image002.pngL) for making multiple dilutions can be identified as

• A) Flask
• B) Micrometers
• C) Cylinder
• D) Micropipettes

Select of the following slide is used for determination of motility?

• A) Glass slide
• B) Microscopic Slide
• C) Permanent Slide
• D) Glass cavity slide

The cell cultures and the fungi growing at the base of the Petri dish may be viewed directly under a type of microscope. Select microscope name.

• A) Inverted microscope
• B) Ultraviolet light microscope
• C) Fluorescent microscope
• D) Bright field microscope

To focus on a specimen, it is best to start with which objective lens?

• A) Intermediate magnification power lens
• B) Lowest magnification power lens
• C) 100X lens
• D) Highest magnification power lens

Which of the following steps is crucial before inoculating bacteria into a liquid culture medium?

• A) Shaking the medium vigorously
• B) Increasing the temperature
• C) Sterilizing the medium
• D) Adding antibiotics

Ebola Virus is life threatening and should be handled at BSL level. Select which BSL level is suitable for Ebola virus?

• A) BSL-3
• B) BSL-1
• C) BSL-4
• D) BSL-2

Which method of sterlization is used for heat sensitive liquids?

• A) Boiling
• B) Pasteurization
• C) Autoclaving
• D) Filteration