The binding between single stranded labeled probe to a complementary nucleotide sequence on the target DNA is called as:

• A) Amplification
• B) Electrophoresis
• C) Restriction digestion
• D) Hybridization

Which of the following statement about adaptor is true?

• A) Chemically synthesized adaptor molecules have a preformed cohesive end that can be used to insert blunt ended foreign DNA into vector
• B) Chemically synthesized adaptor molecules are blunt ended that can be used to insert cohesive ended foreign DNA into vector
• C)
• D)

Southern blot is used to detect the presence of a particular piece of DNA in a sample by:

• A) Labeled probe
• B) Unlabeled probe
• C) Primer pair
• D) Labeled protein

DNA is extracted from cells, called ________

• A) Thrombocytes
• B) Leukocytes
• C) Plasma
• D) Erythrocytes

The Southern blot is used to detect the presence of a particular piece of _________ in a sample by a molecular probe.

• A) DNA
• B) RNA
• C) Proteins
• D) Carbohydrate

When a linearized plasmid vector DNA is treated with alkaline phosphatase it will results in:

• A) Removal of 3’-terminal phosphate groups that will prevent recircularization and plasmid dimer formation
• B) Removal of 5’-terminal phosphate groups that will prevent recircularization and plasmid dimer formation
• C)
• D)

Which type of membrane is used for analysis of the DNA fragments during Southern blotting?

• A) Both nitrocellulose and nylon
• B) Nylon
• C) Nitrocellulose
• D) Gel only

Which of the following enzyme is used for the removal of single phosphate groups from 5´-ends of linear vectors?

• A) Alkaline Phosphatase
• B) DNA ligase
• C) RNA ligase
• D) DNA Polymerase

Which of the following methods is not used to join DNA in vitro?

• A) Disjoining blunt-ended fragments by DNA ligase from phage T4 infected E. coli
• B) Joining covalently annealed cohesive ends by DNA ligase
• C) Utilizing the enzyme terminal deoxynucleotidyltransferase to synthesize homopolymeric 3’ single stranded tails at the ends of fragments
• D) Joining blunt-ended fragments by DNA ligase from phage T4 infected E. coli

The DNA fragments are separated on the basis of ________by electrophoresis.

• A) Size
• B) Charge
• C) Speed
• D) Shape

The process of detection of the bands complementary to the probe, during Southern blotting is called as ________.

• A) Autobiography
• B) Biography
• C) Autography
• D) Autoradiography

Alkaline phosphatase is an enzyme responsible for removing _______ groups from many types of molecules including DNA.

• A) Phosphate
• B) Amino
• C) Kinase
• D) Carboxyl

To identify DNA sequence or mutation, _______ blotting can be used.

• A) Southern
• B) Northern
• C) Eastern
• D) Western

T4 DNA ligase is used to join _____ DNA molecules.

• A) Cohesive ended
• B) Blunt-ended
• C)
• D)

Which of the following are the transfer methods of DNA to membrane during southern blotting?

• A) Electrophoretic transfer
• B) All of the options
• C) Simultaneous transfer to two membranes
• D) Vacuum transfer

Which type of probes enable autoradiographic detection during Southern blotting?

• A) Non radioactive
• B) Charged
• C) Radioactive
• D) Inert

Labeled material to detect complementary region in the gene or DNA is called as.

• A) DNTPs
• B) Blocking agents
• C) Biotin
• D) Probe

The presence of a mutation affecting a restriction site causes the pattern of bands to ______ from those seen in a normal gene.

• A) Differ
• B) Same
• C) Not affected
• D) All of the options

E. coli and phage T4 encode an enzyme which seals single stranded nicks between adjacent nucleotides in a duplex DNA chain known as:

• A) None of the options
• B) DNA ligase
• C) Topoisomerase
• D) Kinase

DNA is cleaved into many fragments by restriction enzyme e.g, ___________.

• A) Helicase
• B) BamH1, EcoR1
• C) Taq polymerase
• D) Polymerase I,II,III