MCQ Bank
In electrophoresis which DNA will move faster towards end
- A) 300bp
- B) 700bp
- C) 100bp
- D) 1Kb
Which of the following is an essential step to break the cell membrane during DNA isolation?
- A) Centrifugation
- B) Homogenization
- C) Precipitation
- D) Resuspension
Why is RNase used in the CTAB-based DNA extraction from plant tissue?
- A) To increase DNA yield
- B) To prevent RNA contamination
- C) To enhance RNA yield
- D) To prevent protein contamination
Which of the following is electrophoresis buffer for DNA gel electrophoresis?
- A) EDTA
- B) Trizol
- C) TNE
- D) TAE
Under an electric field, in which direction DNA travels in agarose gel?
- A) -ive to +ive
- B) +ive to -ive
- C) Can travel in both directions
- D) Electric field does not impact DNA movement in gel
What is the purpose of washing the DNA pellet with ethanol in the final steps?
- A) To isolate DNA from solution
- B) To remove remaining cell debris
- C) To improve DNA stability
- D) To increase DNA yield
Which of the following nitrogenous base is not present in DNA?
- A) Adenine
- B) Uracil
- C) Cytosine
- D) Thymine
CTAB used in the DNA extraction from plant tissue, stands for?
- A) Cellulose Trisodium Absorption Buffer
- B) Cetyl-trimethyl-ammonium Bromide
- C) Cellular Tissue Absorption Buffer
- D) Chloroplast Treatment and Absorption Base
If we have DNA fragments of the following sizes, which fragment in gel electrophoresis will move faster?
- A) 500 bp
- B) 1000 bp
- C) 100 bp
- D) 1 kb
Which component helps separate DNA from cellular proteins and other contaminants during extraction method?
- A) Ethanol
- B) Isopropanol
- C) Lysis buffer
- D) Phenol-chloroform
To make cDNA, which enzyme is used?
- A) Ligase
- B) DNA polymerase
- C) Reverse transcriptase
- D) RNA polymerase