What is the purpose of washing the DNA pellet with ethanol during DNA isolation?

• A) To increase DNA concentration
• B) To increase DNA stability
• C) To promote protein binding with DNA
• D) To remove the residual contaminations

RNA is stored at

• A) 4ºC
• B) Room temperature
• C) 30ºC
• D) -80ºC

Why is heat shock step used in the transformation of competent cells?

• A) To induce cell lysis
• B) To enhance protein expression
• C) To kill bacterial contaminants
• D) To facilitate DNA uptake by the cells

One of the advantages of real-time PCR (qPCR) is:

• A) Quantification during the exponential phase
• B) Reduced reaction time
• C) Increased specificity
• D) Increased sensitivity

For the transformation in competent cells, a heat shock at _________ is given to the cells.

• A) 42 ºC
• B) 38 ºC
• C) 50 ºC
• D) 65 ºC

The assembly difference between DNA gel and SDS-PAGE is?

• A) DNA gel is vertical
• B) PAGE is horizontal
• C) There is no difference
• D) PAGE is vertical

----------- is the technique which is mendatory for RFLP analysis?

• A) Gel electrophoresis
• B) qPCR
• C) Northern blotting
• D) SDS page

cDNA synthesis in molecular biology is done for the purpose of?

• A) To amplify the RNA
• B) To make RNA from DNA
• C) To make complementary DNA form RNA
• D) To amplify the DNA

Which of the following factor determines the movement of protein in PAGE anlaysis?

• A) Structure of the protein
• B) Size of the protein
• C) Shape of the protein
• D) Concentration of protein

In SDS-PAGE, the protein runs under the influence of?

• A) Magnetic field
• B) All of the above
• C) Electromagnetic field
• D) Electric field

If we are doing the manual isolation of plasmid by alkaline lysis method, EDTA will be used in?

• A) Final dilution of pellet
• B) Solution II
• C) Solution III
• D) Solution I

Which fluorescence method is commonly used to monitor the qRT-PCR reaction?

• A) Bromophenol blue
• B) Ethidium bromide staining
• C) Crystal Violet
• D) SYBR Green

Plasmid DNA is mostly used in -------?

• A) Recombinant DNA technology
• B) AFLP analysis
• C) RFLP anlaysis
• D) Proteomics studies

The process of cDNA yields?

• A) One strand of DNA and one strand of RNA
• B) Double stranded RNA
• C) Single-stranded DNA
• D) Double stranded DNA copy of RNA

Basic requirements for the Polymerase chain reaction involve: A: Template for amplification B: DNA polymerase C: Primers

• A) A, B and C
• B) B only
• C) C only
• D) A and B only

By using the technique PCR we can detect______?

• A) Absence of a gene
• B) All of the above
• C) Presence of a gene
• D) Expression of a gene

What does qRT-PCR stand for?

• A) Quantitative Real-time PCR
• B) Quantitative Reverse Transcription PCR
• C) Quality Replication Time PCR
• D) Quantitative Rapid Time PCR

If there is no availability of elution buffer, what could be the alternative to dissolve the plasmid DNA pellet?

• A) Water
• B) EDTA
• C) TAE buffer
• D) Ethanol

What is the source of DNA used in DNA fingerprinting?

• A) All of the above
• B) Hair
• C) Blood
• D) Saliva

If we forgot to add Taq polymerase in a PCR reaction, what could be the possible outcome of reaction?

• A) Amplification of non-specific DNA fragments
• B) Primers will not bind to DNA
• C) No amplification
• D) pH of the reaction mixture will be changed