Which of the following is the basic requirement of PCR reaction?

• A) All of the given options
• B) Heat-stable DNA polymerase
• C) DNA segment to be amplified
• D) Oligonucleotide primers

The quick quantitative method for measuring antigen by precipitation is _____ .

• A) Solid phase radio immunoassay
• B) Immunodiffusion
• C) ELISA
• D) Fluorescent microscopy

The PCR technique was developed by_________.

• A) Altman
• B) Milstein
• C) Kary Mullis
• D) Kohler

Which of the following is an immunodiffusion test?

• A) All of the given options
• B) Gel diffusion
• C) Ouchterloney technique
• D) Double-diffusion

Reverse transcriptase is responsible for the conversion of ______ into _____.

• A) Proteins, RNA
• B) cDNA, RNA
• C) RNA, cDNA
• D) RNA, Proteins

Which of the following molecule(s) can be detected by ELISA?

• A) Hormones
• B) Antibodies
• C) All of the above
• D) Proteins

Which one of the following is a bacteriophage?

• A) Pox virus
• B) M13
• C) Retrovirus
• D) TMV

The process of binding of primer to the denatured strand is called as____ .

• A) Denaturation
• B) Renaturation
• C) Extension
• D) Annealing

Which one of the following is true regarding Oakley Fulthorpe procedure?

• A) Double diffusion in one dimension
• B) Double diffusion in two dimensions
• C) Single diffusion in two dimension
• D) Single diffusion in one dimension

The genome of bacteriophages can be _____ .

• A) Either DNA or RNA
• B) DNA only
• C) Both DNA and RNA
• D) RNA only

Viruses can be precipitated by using_____ .

• A) DNA inhibitor
• B) Ethanol
• C) Ethyl Ether
• D) Chloroform

Filter having the pore size of ____ µm could remove bacterial contamination from sample

• A) 0.1-1
• B) 0.2-2
• C) 0.3-1.1
• D) 1-10

Which one of the following is an application of Vacuum filtration technique?

• A) Collection of a desired solid
• B) Removal of impurities
• C) Viral size determination
• D) Retention of large viruses

Which one of the following substances is used to determine the type of genome present in viruses?

• A) Ethyl ether
• B) Thymidine kinase
• C) DNA inhibitor
• D) Chloroform

Which one of the following viruses do not produce cytopathic effects?

• A) Hepatitis C virus
• B) Herpes Simplex Virus
• C) Enterovirus
• D) Coxsackievirus

Which of the following is immobilized on the microtiter well in sandwich ELISA?

• A) Detection antibody
• B) Sample
• C) Secondary antibody conjugated to an enzyme
• D) Capture antibody

Thermus aquatics is the source of _________.

• A) DNA Polymerase
• B) Taq polymerase
• C) Vent polymerase
• D) Primase enzyme

Which one of the following viral component assembles first, during bacteriophage assembly inside host cell?

• A) Base plates
• B) Capsid
• C) Envelope
• D) Tail

Primers used for the process of polymerase chain reaction are _________.

• A) Single-stranded DNA oligonucleotide
• B) Single-stranded RNA oligonucleotide
• C) Double-stranded DNA oligonucleotide
• D) Double-stranded RNA oligonucleotide

Denaturation is the process of _________.

• A) Heating above 100°C
• B) Heating between 90 to 98°C
• C) Heating between 40 to 60°C
• D) Heating between 72°C