Vectors that can replicate and are maintained in two or more unrelated host organisms are called ____vectors

• A) expression
• B) shuttle
• C) host
• D) house keeping

Expression from a strong promoter can represent ________ of cloned gene product of total cell protein

• A) 20-50%
• B) 30-40%
• C) 20-40%
• D) 10-40%

YACs are the ______ used for large DNA fragments insertion

• A) Yeast artificial chromosomes
• B) Y-cloned artificial chromosomes
• C) Yok artificial chromosomes
• D) Y-linked autosomal clones

Foreign proteins in E. coli is

• A) submerged
• B) Stable
• C) Dissolved
• D) Instable

Vectors that can replicate and are stably maintained in two or more unrelated host organisms are called ____

• A) internal vectors
• B) shuttle vectors
• C) target vectors
• D) host specific vectors

A great advantage of hybridization for library screening is that it is

• A) Difficult
• B) Good
• C) Versatile
• D) Unique

It is much more convenient to use ________________, because these have a higher capacity and the efficiency of in vitro packaging

• A) bacteriophage simple insertion vectors
• B) Bacteriophage-ʎ insertion vectors
• C) Bacteriophage-ECol I insertion vectors
• D) Bacteriophage-ʎ deletion vectors

Gram-negative bacteria such as E. coli have a

• A) complex wall-membrane mitochondia
• B) Complex wall-membrane
• C) Difficult wall-membrane
• D) Simple wall-membrane

This is the process by which a particular DNA sequence compensates for a missing function in a mutant cell and thus restores the wild type phenotype.

• A) Functional complementation
• B) Functional genomics
• C) Non-Functional complementation
• D) Functional analysis

________ with specific primers could be used to isolate genes directly from genomic DNA

• A) SNPs
• B) PCR
• C) AFLP
• D) RFLP

Bacterial artificial chromosomes are used as_____

• A) plasmid recombinant vectors
• B) cloning vectors
• C) restriction enzymes
• D) protein docking molecules

_______ are the plasmids containing a fragment of ʎ DNA including ‘cos’ site

• A) phasmid
• B) M13
• C) none of these
• D) cosmid

In chromosome jumping, the DNA of interest is identified, cut into fragments with restriction enzymes and

• A) Breaks down
• B) Circularized
• C) Straighten
• D) Elongates

In Genomic DNA libraries, the appropriately sized random fragments can be produced by ______

• A) polymerases
• B) ligases
• C) lysozymes
• D) restriction endonucleases

In South-western and north-western blotting screening is carried out without using an antibody, by incubating the membranes with

• A) Radiolabelled single stranded DNA probe
• B) Unlabelled double stranded DNA probe
• C) Radiolabelled double stranded DNA probe
• D) Radiolabelled double stranded RNA probe

________ vectors are combination of plasmid and ʎ phage sequences

• A) M13
• B) Phasmid
• C) Cosmid
• D) Chloroplast

M13 is a filamentous _________ with single stranded DNA that replicates without killing its host

• A) mitochondrial
• B) chloroplast
• C) bacteriophage
• D) plasmid

To isolate specific clone, PCR is carried out with gene-specific primers

• A) universal primers
• B) simple primers
• C) special primers
• D) gene-specific primers

Benton and Davis (1977) devised a method called

• A) Plaque up
• B) Plaque lift
• C) Plaque down
• D) Plaque rite

Chromosome Walking, In human genome, the starting point may be

• A) Cleaved Amplified Polymorphic Sequence (CAPS)
• B) restriction fragment length polymorphism (RFLP)
• C) Amplified fragment length polymorphism (AFLP)
• D) single-nucleotide polymorphisms (SNPs)