MCQ Bank
Primers used for the process of polymerase chain reaction are
- A) Double-stranded RNA oligonucleotide
- B) Double-stranded DNA oligonucleotide
- C) Single-stranded RNA oligonucleotide
- D) Single-stranded DNA oligonucleotide
In Nested PCR, second PCR product is -----------than the first one
- A) Both (b) and (c)
- B) Shorter
- C) Longer
- D) Equal length
Two pair of enzymes are used in PCR
- A) RT-PCR
- B) Nested
- C) Conventional
- D) Multiplex
Two sets of primers instead of one pair are uses in --------------------
- A) None of these
- B) In-situ PCR
- C) Traditional PCR
- D) Nested PCR
What are the base-pairing rules in DNA?
- A) A-C. G-U
- B) A-C, G-T
- C) A-U, G-C
- D) A-T.G-C
In is a polymerase chain reaction that actually takes place inside the cell on a slide
- A) Colony
- B) Nested PCR
- C) Situ PCR (ISH)
- D) RT-PCR
The extension rate of Taq Polymerase enzyme is --------------------
- A) 65 nt/sec
- B) 75 nt/sec
- C) 60 nt/sec
- D) 70 nt/sec
How many DNA duplexes are obtained from one DNA duplex after 4 cycles of PCR?
- A) 4
- B) 6
- C) 32
- D) 16
DNA fragments separated on an agarose gel can be visualized after staining with
- A) Aniline blue
- B) Ethidium bromide
- C) Bromophenol blue
- D) Acetocarmine
Which of the following is the basic requirement of PCR reaction?
- A) DNA segment to be amplified
- B) All of the above
- C) A heat-stable DNA polymerase
- D) Two oligonucleotide primers
Which of the following is the basic requirement of PCR reaction?
- A) DNA segment to be amplified
- B) All of the above
- C) A heat-stable DNA polymerase
- D) Two oligonucleotide primers
----enzymes have symmetrical recognition sequences but are of little value
- A) TypeIII
- B) Exonucleases
- C) TypeII
- D) TypeI
-------------is used for the screening of bacterial or yeast clones for correct ligation or plasmid products
- A) RT-PCR
- B) Real time PCR
- C) Nested PCR
- D) Colony PCR
AFLP is a highly sensitive PCR-based method for detecting in DNA
- A) Polymorphisms
- B) Deletion
- C) Insertion
- D) Duplication
Agarose extracted from sea weeds finds use in-----
- A) Spectrophotometry
- B) Gel electrophoresis
- C) PCR
- D) Tissue culture
------------PCR uses the template for the reverse primers is a restriction fragment that has been self-ligated
- A) Inverse
- B) Allele-specific
- C) Colony
- D) Asymmetric
Which of the following statements are true regarding PCR?
- A) Primer extension occurs at 72°C
- B) Denaturation involves heating at 90 to 98°C
- C) Annealing involves the binding of primer between 40 to 60C°C
- D) All of the above
tools have also allowed to perform prenatal genetic diagnosis
- A) Molecular
- B) Microbiology
- C) Histology
- D) Radiology
The extension rate of Taq Polymerase enzyme is --------------------
- A) 60 nt/sec
- B) 65 nt/sec
- C) 70 nt/sec
- D) Not clear
What would the generally expected effect on the PCR reaction be of adjustments that increase the temperature of the annealing phase and the length of the elongation phase?
- A) Precision and yield will be reduced
- B) Precision will be reduced, but yield will be increased
- C) Precision will be increased, but yield will be reduced
- D) Precision and yield will be increased