PCR uses the template for the reverse primers is a restriction fragment that has been self-ligated.

• A) Inverse
• B) Allele-specific
• C) Asymmetric
• D) Colony

In------ PCR there is very low probability of non-specific amplification of DNA

• A) Conventional
• B) Multiplex
• C) Nested
• D) RT-PCR

Allele-specific PCR uses primers whose ___ encompass the SNP

• A) 3' ends
• B) None
• C) Promoter region
• D) 5' ends

----------consists of multiple primer sets within a single PCR mixture to produce amplicons of varying sizes.

• A) Multiplex-PCR
• B) Nested PCR
• C) All given
• D) RT-PCR

___ tools have also allowed to perform prenatal genetic diagnosis

• A) Microbiology
• B) Histology
• C) Radiology
• D) Molecular

Synthesis of cDNA takes place using oligo dT primers at

• A) (37°C) one hour -
• B) (40°C) two hour
• C) (25°C) one hour -
• D) (18°C) one hour -

are able to initiate replication from a large number of target locations

• A) None
• B) Specific Primers
• C) Degenerate Primers
• D) Reverse Primers

A heat stable enzyme called Taq Polymerase is used in Polymerase Chain Reaction (PCR) which is obtained from a bacterium ------------=

• A) Escherichia coli
• B) Actinobacillus capsulatus
• C) Thermus aquaticus
• D) Salmonella Typhi

-----is used to clone sequences flanking a known sequence.

• A) Nested PCR
• B) Inverse PCR
• C) None of these
• D) Real Time PCR

AFLP is a highly sensitive PCR-based method used for detecting polymorphisms in

• A) None of these
• B) RNA
• C) DNA
• D) Both (a) and (b)

Distance between the primers binding sites will determine the -----------of PCR product

• A) Thickness
• B) Nature
• C) Molecular weight
• D) Size

A fixed cell or tissue can be amplified by type of PCR

• A) Nested PCT
• B) In situ
• C) Colony PCR
• D) RT-PCT

What would be the effect on the PCR reaction if any of the following circumstances arose: 1) there are no primers in the reaction, 2) there are no dNTPs in the reaction, 3) there is no Taq polymerase in the reaction?

• A) Non-specific PCR of random templates will occur
• B) PCR would proceed normally
• C) The reaction will cease after a few cycles
• D) The PCR reaction will not commence

Why are vent polymerase and Pfu more efficient than the Taq polymerase?

• A) None of the above
• B) Because of more efficient polymerase activity
• C) Because of proofreading activity
• D) Both

For the extension step of PCR. the temperature is set at ------------=

• A) 65°C
• B) 72°C
• C) 94°C
• D) 55 °C

Molecular Detection methods can facilitate in detection of -------in plants, animals and environment

• A) Pathogens
• B) radioactive elements
• C) trace element
• D) pigments

-----PCR uses primers whose 3' ends encompass the SNP.

• A) Allele Specific PCR
• B) Ligation mediated PCR
• C) Reverse Transcriptase PCR
• D) Ligation mediated PCR

The process of reverse transcription reverse transcribes

• A) DNA into DNA
• B) DNA into RNA
• C) RNA into DNA
• D) All given

A technique for making millions of copies of a specific region of DNA.

• A) Restriction enzymes
• B) DNA ligase
• C) Gel electrophoresis
• D) Polymerase chain reaction

Melting of DNA template results in yielding of DNA

• A) All given
• B) Replicated strands
• C) Double strands
• D) Single strands