MCQ Bank
In is a polymerase chain reaction that actually takes place inside the cell on a slide
- A) Colony
- B) Nested PCR
- C) Situ PCR (ISH)
- D) RT-PCR
The extension rate of Taq Polymerase enzyme is --------------------
- A) 65 nt/sec
- B) 75 nt/sec
- C) 60 nt/sec
- D) 70 nt/sec
How many DNA duplexes are obtained from one DNA duplex after 4 cycles of PCR?
- A) 4
- B) 6
- C) 32
- D) 16
DNA fragments separated on an agarose gel can be visualized after staining with
- A) Aniline blue
- B) Ethidium bromide
- C) Bromophenol blue
- D) Acetocarmine
Which of the following is the basic requirement of PCR reaction?
- A) DNA segment to be amplified
- B) All of the above
- C) A heat-stable DNA polymerase
- D) Two oligonucleotide primers
Which of the following is the basic requirement of PCR reaction?
- A) DNA segment to be amplified
- B) All of the above
- C) A heat-stable DNA polymerase
- D) Two oligonucleotide primers
----enzymes have symmetrical recognition sequences but are of little value
- A) TypeIII
- B) Exonucleases
- C) TypeII
- D) TypeI
-------------is used for the screening of bacterial or yeast clones for correct ligation or plasmid products
- A) RT-PCR
- B) Real time PCR
- C) Nested PCR
- D) Colony PCR
AFLP is a highly sensitive PCR-based method for detecting in DNA
- A) Polymorphisms
- B) Deletion
- C) Insertion
- D) Duplication
Agarose extracted from sea weeds finds use in-----
- A) Spectrophotometry
- B) Gel electrophoresis
- C) PCR
- D) Tissue culture
------------PCR uses the template for the reverse primers is a restriction fragment that has been self-ligated
- A) Inverse
- B) Allele-specific
- C) Colony
- D) Asymmetric
Which of the following statements are true regarding PCR?
- A) Primer extension occurs at 72°C
- B) Denaturation involves heating at 90 to 98°C
- C) Annealing involves the binding of primer between 40 to 60C°C
- D) All of the above
tools have also allowed to perform prenatal genetic diagnosis
- A) Molecular
- B) Microbiology
- C) Histology
- D) Radiology
The extension rate of Taq Polymerase enzyme is --------------------
- A) 60 nt/sec
- B) 65 nt/sec
- C) 70 nt/sec
- D) Not clear
What would the generally expected effect on the PCR reaction be of adjustments that increase the temperature of the annealing phase and the length of the elongation phase?
- A) Precision and yield will be reduced
- B) Precision will be reduced, but yield will be increased
- C) Precision will be increased, but yield will be reduced
- D) Precision and yield will be increased
The instrument used to run the PCR is known as - =
- A) Centrifuge machine
- B) Incubator
- C) UV Transilluminator
- D) Thermocycler
Non genomic material such as RNA can be detected by
- A) in situ PCR
- B) Conventional PCR
- C) Western blotting
- D) Spectrophotometer
Fraternal twins are the result of _____ separate fertilization events
- A) Three
- B) Single
- C) Two
- D) Four
Neurulation in ______ is exclusively secondary.
- A) Mammales
- B) Reptiles
- C) Fishes
- D) Amphibians
discoidal meroblastic cleavage occur in ________.
- A) none of these
- B) birds and reptiles
- C) amphibians
- D) mammals