BT403 Subjective Paper 3 marks question: Q1:Write down the limitation of PEG mediated transformation? Q2:Why pesticides affect the kids more as compared to adults? Q3:What is pre-zygotic barriers? 5 marks question: Q4:What is tissue culture? (a) What are major steps of tissue culture. (b) Which media is used to facilitate this process. Q5: How crown gall diseases occur? Q6: Write doen the mechanisms of resistance in plants?