The assembly difference between DNA gel and SDS-PAGE is?

• A) DNA gel is vertical
• B) PAGE is horizontal
• C) There is no difference
• D) PAGE is vertical

----------- is the technique which is mendatory for RFLP analysis?

• A) Gel electrophoresis
• B) qPCR
• C) Northern blotting
• D) SDS page

cDNA synthesis in molecular biology is done for the purpose of?

• A) To amplify the RNA
• B) To make RNA from DNA
• C) To make complementary DNA form RNA
• D) To amplify the DNA

Which of the following factor determines the movement of protein in PAGE anlaysis?

• A) Structure of the protein
• B) Size of the protein
• C) Shape of the protein
• D) Concentration of protein

In SDS-PAGE, the protein runs under the influence of?

• A) Magnetic field
• B) All of the above
• C) Electromagnetic field
• D) Electric field

If we are doing the manual isolation of plasmid by alkaline lysis method, EDTA will be used in?

• A) Final dilution of pellet
• B) Solution II
• C) Solution III
• D) Solution I

Which fluorescence method is commonly used to monitor the qRT-PCR reaction?

• A) Bromophenol blue
• B) Ethidium bromide staining
• C) Crystal Violet
• D) SYBR Green

Plasmid DNA is mostly used in -------?

• A) Recombinant DNA technology
• B) AFLP analysis
• C) RFLP anlaysis
• D) Proteomics studies

The process of cDNA yields?

• A) One strand of DNA and one strand of RNA
• B) Double stranded RNA
• C) Single-stranded DNA
• D) Double stranded DNA copy of RNA

Basic requirements for the Polymerase chain reaction involve: A: Template for amplification B: DNA polymerase C: Primers

• A) A, B and C
• B) B only
• C) C only
• D) A and B only

By using the technique PCR we can detect______?

• A) Absence of a gene
• B) All of the above
• C) Presence of a gene
• D) Expression of a gene

What does qRT-PCR stand for?

• A) Quantitative Real-time PCR
• B) Quantitative Reverse Transcription PCR
• C) Quality Replication Time PCR
• D) Quantitative Rapid Time PCR

If there is no availability of elution buffer, what could be the alternative to dissolve the plasmid DNA pellet?

• A) Water
• B) EDTA
• C) TAE buffer
• D) Ethanol

What is the source of DNA used in DNA fingerprinting?

• A) All of the above
• B) Hair
• C) Blood
• D) Saliva

If we forgot to add Taq polymerase in a PCR reaction, what could be the possible outcome of reaction?

• A) Amplification of non-specific DNA fragments
• B) Primers will not bind to DNA
• C) No amplification
• D) pH of the reaction mixture will be changed

What is the function of reverse transcriptase during cDNA synthesis?

• A) Synthesizing RNA from DNA
• B) Joining DNA fragments
• C) Replicating DNA strands
• D) Synthesizing DNA from RNA

What does cDNA stand for in the context of molecular biology?

• A) Complementary DNA
• B) Chromosomal DNA
• C) Cellular DNA
• D) Circular DNA

Which of the following PCR technique can be used to quantify gene expression?

• A) Reverse Transcription PCR (RT-PCR)
• B) Hot start PCR
• C) Multiplex PCR
• D) Quantitative PCR (qPCR)

In which direction DNA is amplified during the PCR?

• A) 5’→3’direction
• B) None of the above
• C) Both A and B
• D) 3’→5’direction

The size of ideal primers for a PCR reaction is?

• A) 18-24 bp
• B) 24-30 bp
• C) 10-15 bp
• D) 15-20 bp